Tg(Dcx-cre/ERT2)DC-F18Zqx
Transgene Detail

Summary

• Symbol: Tg(Dcx-cre/ERT2)DC-F18Zqx
• Name: transgene insertion DC-F18, Zhi-Qi Xiong
• MGI ID: MGI:5438982
• Synonyms: DC-F18, Dcx-CreER^T2
• Transgene: Tg(Dcx-cre/ERT2)DC-F18Zqx Location: unknown
• Alliance: Tg(Dcx-cre/ERT2)DC-F18Zqx page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Dcx-cre/ERT2)DC-F18Zqx expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dcx (MGI:1277171)

• Mutation details: A BAC clone (RP23-462G16) containing the Dcx locus as well as 30 kb upstream and 60 kb downstream was homologously recombined with a targeting vector to insert the cre/ERT2 open reading frame, a bovine growth hormone polyA tag and an Frt-flanked neo marker into exon 2 of Dcx which silenced the BAC copy of Dcx. Arabinose-induced flp expression in bacterial cells removed the neo cassette from the construct. The linearized, purified construct was microinjected into pronuclei of fertilized C57BL/6 eggs. Founders were crossed to cre-responsive Rosa26-lacz mice to identify animals with the neural expression of cre. Line DC-F18 showed tamoxifen-induced cre activity in the subgranular zone (SGZ) of the dentate gyrus, but not in the subventricular zone (SVZ), with non-specific labeling in some neurons of the piriform cortex, amygdala, and hypothalamus also observed. This line was analyzed in further detail in the SGZ. (J:187505)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dcx (mouse) Summary of all recombinase alleles driven by Dcx. Alliance mouse Dcx gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:187505 Cheng X, et al., Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone. Cell Res. 2011 Feb;21(2):338-49
• All: 9 reference(s)