Dag1^tm1.1Swin
Targeted Allele Detail

Summary

• Symbol: Dag1^tm1.1Swin
• Name: dystroglycan 1; targeted mutation 1.1, Steve J Winder
• MGI ID: MGI:5438989
• Synonyms: Dag1^Y890F
• Gene: Dag1 Location: Chr9:108082060-108141176 bp, - strand Genetic Position: Chr9, 59.08 cM
• Alliance: Dag1^tm1.1Swin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187750
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted upstream of exon 2. Exon 2 was replaced with a modified version in which a nucleotide substitution (A to T) results in the amino acid substitution of tyrosine with phenylalanine at position 890 (Y890F). Subsequent cre-mediated recombination removed the neo cassette. (J:187750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dag1 Mutation: 109 strains or lines available

References

• Original: J:187750 Miller G, et al., Preventing phosphorylation of dystroglycan ameliorates the dystrophic phenotype in mdx mouse. Hum Mol Genet. 2012 Oct 15;21(20):4508-20
• All: 1 reference(s)