Tg(Dcx-cre/ERT2)DC-F30Zqx
Transgene Detail

Summary

• Symbol: Tg(Dcx-cre/ERT2)DC-F30Zqx
• Name: transgene insertion DC-F30, Zhi-Qi Xiong
• MGI ID: MGI:5438991
• Synonyms: DC-F30, Dcx-CreER^T2
• Transgene: Tg(Dcx-cre/ERT2)DC-F30Zqx Location: unknown
• Alliance: Tg(Dcx-cre/ERT2)DC-F30Zqx page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Dcx-cre/ERT2)DC-F30Zqx expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dcx (MGI:1277171)

• Mutation details: A BAC clone (RP23-462G16) containing the Dcx locus as well as 30 kb upstream and 60 kb downstream was homologously recombined with a targeting vector to insert the cre/ERT2 open reading frame, a bovine growth hormone polyA tag and an Frt-flanked neo marker into exon 2 of Dcx which silenced the BAC copy of Dcx. Arabinose-induced flp expression in bacterial cells removed the neo cassette from the construct. The linearized, purified construct was microinjected into pronuclei of fertilized C57BL/6 eggs. In a cross to Rosa26-YFP reporter mice, line DC-F30 showed robust tamoxifen-induced ectopic YFP labeling in layer III. IV, and VI of the cortex with some of these neurons from these layers projecting to the dentate gyrus through the perforant pathway. Labeling of cortical axons allowed delineation of molecular layer dendritic fields and facilitated analysis of spine differentiation in the inner molecular layer. (J:187505)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dcx (mouse) Summary of all recombinase alleles driven by Dcx. Alliance mouse Dcx gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:187505 Cheng X, et al., Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone. Cell Res. 2011 Feb;21(2):338-49
• All: 4 reference(s)