Actb^{tm1.1(tetO-COP4*/EYFP)Kftnk}
Targeted Allele Detail

Summary

• Symbol: Actb^{tm1.1(tetO-COP4*/EYFP)Kftnk}
• Name: actin, beta; targeted mutation 1.1, Kenji Tanaka
• MGI ID: MGI:5439159
• Synonyms: tetO-ChR2(C128S)-EYFP
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{tm1.1(tetO-COP4*/EYFP)Kftnk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186771
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6 x 129

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Null/knockout, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: An expression cassette containing a tetracycline responsive element that drives inducible expression of a channelrhodopsin-2 (with a C128S mutation) and EYFP fusion was inserted after the endogenous polyadenylation sequence. Flp-mediated recombination removed an FRT-flanked neo cassette. (J:186771)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

Notes

ES cell = G0G1 (C57BL/6 x 129)

References

• Original: J:186771 Tanaka KF, et al., Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system. Cell Rep. 2012 Aug 30;2(2):397-406
• All: 27 reference(s)