Spaca1^tm1.1Osb
Targeted Allele Detail

Summary

• Symbol: Spaca1^tm1.1Osb
• Name: sperm acrosome associated 1; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:5439282
• Gene: Spaca1 Location: Chr4:34024872-34050067 bp, - strand Genetic Position: Chr4, 16.63 cM, cytoband A5
• Alliance: Spaca1^tm1.1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187685
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and inverted FRT flanked neo cassette were inserted upstream of exon 3 and a loxP site was inserted downstream of exon 3 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 3 and 4. (J:187685)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spaca1 Mutation: 16 strains or lines available

Notes

EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb.

References

• Original: J:187685 Fujihara Y, et al., SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia. Development. 2012 Oct;139(19):3583-9
• All: 1 reference(s)