Htr1a^tm3.1Rhn
Targeted Allele Detail

Summary

• Symbol: Htr1a^tm3.1Rhn
• Name: 5-hydroxytryptamine (serotonin) receptor 1A; targeted mutation 3.1, Rene Hen
• MGI ID: MGI:5439287
• Synonyms: Htr1a^tetO, Htr1A tetO
• Gene: Htr1a Location: Chr13:105580147-105584630 bp, + strand Genetic Position: Chr13, 56.92 cM
• Alliance: Htr1a^tm3.1Rhn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137439
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Flexible Accelerated STOP Tetracycline Operator (tetO)-knockin (FAST) was used to insert a construct containing a loxP site, FRT site, neo cassette, STOP, FRT site, tetracycline operator (tetO) and loxP site was inserted upstream of the translation initiation site. Flp-mediated recombination removed the neo/STOP cassette and left the tetO floxed and intact. Autoradiography confirmed a 20% reduction in protein expression. (J:137439, J:167659)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Htr1a Mutation: 26 strains or lines available

References

• Original: J:137439 Audero E, et al., Sporadic autonomic dysregulation and death associated with excessive serotonin autoinhibition. Science. 2008 Jul 4;321(5885):130-3
• All: 9 reference(s)