Tg(Klf4-cre/ERT)1Wai
Transgene Detail

Summary

• Symbol: Tg(Klf4-cre/ERT)1Wai
• Name: transgene insertion 1, Walden Ai
• MGI ID: MGI:5439626
• Synonyms: KLF4/CreER^TM
• Transgene: Tg(Klf4-cre/ERT)1Wai Location: unknown
• Alliance: Tg(Klf4-cre/ERT)1Wai page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Klf4-cre/ERT)1Wai expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Klf4 (MGI:1342287)

• Mutation details: A BAC clone containing the murine Klf4 gene was modified by recombineering methods so that a cre recombinase sequence was fused to a tamoxifen-inducible estrogen receptor was inserted at the initial methionine (start codon). The purified construct was injected into fertilized oocytes. Founder animals were generated and cre activity was analyzed by crossing founders to the cre-responsive reporter (ROSA-lacZ) or floxed Klf4 animals. (J:187932, J:188101, J:189337)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Klf4 (mouse) Summary of all recombinase alleles driven by Klf4. Comparative matrix view of recombinase activities Alliance mouse Klf4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:188101 Yu T, et al., Kruppel-like factor 4 regulates intestinal epithelial cell morphology and polarity. PLoS One. 2012;7(2):e32492
• All: 3 reference(s)