Mcm8^tm1.1Geno
Targeted Allele Detail

Summary

• Symbol: Mcm8^tm1.1Geno
• Name: minichromosome maintenance 8 homologous recombination repair factor; targeted mutation 1.1, Genoway
• MGI ID: MGI:5439837
• Gene: Mcm8 Location: Chr2:132658061-132686117 bp, + strand Genetic Position: Chr2, 64.75 cM, cytoband F3
• Alliance: Mcm8^tm1.1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187623
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT_flanked neo cassette with a 5' loxP site was inserted upstream of exon 11. An additional loxP site was inserted downstream of exon 12. Cre-mediated recombination removed the neo cassette, exon 11 and exon 12. Western blot analysis confirmed the absence of protein expression in testis. (J:187623)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm8 Mutation: 37 strains or lines available

References

• Original: J:187623 Lutzmann M, et al., MCM8- and MCM9-Deficient Mice Reveal Gametogenesis Defects and Genome Instability Due to Impaired Homologous Recombination. Mol Cell. 2012 Aug 24;47(4):523-34
• All: 3 reference(s)