Tg(Adora2a-Chrm3*,-mCherry)AD6Blr
Transgene Detail

Summary

• Symbol: Tg(Adora2a-Chrm3*,-mCherry)AD6Blr
• Name: transgene insertion AD6, Bryan L Roth
• MGI ID: MGI:5440979
• Synonyms: adora2A-rM3Ds, adora2A-rM3Ds-IRES-mCherry
• Transgene: Tg(Adora2a-Chrm3*,-mCherry)AD6Blr Location: unknown
• Alliance: Tg(Adora2a-Chrm3*,-mCherry)AD6Blr page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL/J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Adora2a-Chrm3*,-mCherry)AD6Blr expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Chrm3 (24260)

  Chrm3

  (MGI:88398)

• Mutation details: The RP24-238K3 BAC harboring the adenosine A2a receptor locus (Adora2a), a ~40 kbp sequence proximal to the Adora2a locus (including part of the Specc1l locus) and ~117 kbp of sequence distal to the Adora2a locus (including the Upb1 locus and most of the 1110038D17Rik locus). A construct containing the mutant G protein-coupled receptor (GPCR) sequence, rM3Ds, with N-terminal hemagglutinin tag, an internal ribosome entry site (IRES), and mCherry fluorescent protein cDNA sequence was inserted downstream of the ATG start site of the Adora2a locus on the BAC via homologous recombination/BAC recombineering. The rM3Ds sequence is a Galpha^s-coupled rat muscarinic 3 DREADD (designer receptor exclusively activated by designer drug), also referred to as R-s, rM3/turkey beta1AR chimer DREADD, Gs-DREADD, Gs-D, or D-s. To create the rM3Ds sequence, the wild-type rat muscarinic 3 receptor (Chrm3) sequence was first modified to replace the second and third intracellular loops with the corresponding turkey beta1-adrenergic receptor (ADRB1 or beta1AR) sequences. The second modification introduced, via site-directed mutagenesis, the Y148C and A238G amino acid substitutions that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). Line AD6 was generated. (J:191042)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:191042 Farrell MS, et al., A Galphas DREADD mouse for selective modulation of cAMP production in striatopallidal neurons. Neuropsychopharmacology. 2013 Apr;38(5):854-62
• All: 5 reference(s)