Gjb2^{tm3.1(Gjb1)Kwi}
Targeted Allele Detail

Summary

• Symbol: Gjb2^{tm3.1(Gjb1)Kwi}
• Name: gap junction protein, beta 2; targeted mutation 3.1, Klaus Willecke
• MGI ID: MGI:5441202
• Synonyms: Cx26^{floxlacZ[Cx32]}
• Gene: Gjb2 Location: Chr14:57336059-57342159 bp, - strand Genetic Position: Chr14, 30.1 cM, cytoband D1-E1
• Alliance: Gjb2^{tm3.1(Gjb1)Kwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188626
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Flp-mediated recombination of Gjb2^tm3(Gjb1)Kwi removed the neo cassette. Further cre-mediated recombination is required to remove the floxed lacZ and allow expression of the inserted coding sequence. Western blot analysis confirmed reduced protein expression in the placenta but not skin. (J:188626)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gjb2 Mutation: 22 strains or lines available

References

• Original: J:188626 Dicke N, et al., Peripheral lymphangiogenesis in mice depends on ectodermal connexin-26 (Gjb2). J Cell Sci. 2011 Aug 15;124(Pt 16):2806-15
• All: 2 reference(s)