Eef2^tm1.1Lepp
Targeted Allele Detail

Summary

• Symbol: Eef2^tm1.1Lepp
• Name: eukaryotic translation elongation factor 2; targeted mutation 1.1, Stephen H Leppla
• MGI ID: MGI:5441712
• Synonyms: eEF2^G17R
• Gene: Eef2 Location: Chr10:81012465-81018332 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Eef2^tm1.1Lepp page

Delayed development in Eef2^tm1.1Lepp/Eef2^tm1.1Lepp mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188600
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Hypomorph) Mutations: Insertion, Nucleotide substitutions Mutation details: A Gly to Arg point mutation at position 717 in exon 12 and a new SacII site in exon 12 were introduced and a floxed neo cassette were inserted after exon 14 via homologous recombination. Cre mediated recombination removed the neo cassette. The point mutation eliminates the diphthamide modification on the protein but leaves the diphthamide biosynthesis machinery intact. (J:188600)

Generation of the Eef2^tm1.1Lepp allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eef2 Mutation: 60 strains or lines available

References

• Original: J:188600 Liu S, et al., Diphthamide modification on eukaryotic elongation factor 2 is needed to assure fidelity of mRNA translation and mouse development. Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):13817-22
• All: 2 reference(s)