Tg(LMNA*G608G)HClns
Transgene Detail

Summary

• Symbol: Tg(LMNA*G608G)HClns
• Name: transgene insertion H, Francis Collins
• MGI ID: MGI:5441745
• Transgene: Tg(LMNA*G608G)HClns Location: unknown Genetic Position: Chr4, Syntenic
• Alliance: Tg(LMNA*G608G)HClns page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(LMNA*G608G)HClns expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  LMNA (4000)

  Lmna	(MGI:96794)

• Mutation details: The 164.4 kb human BAC RP11-702H12 containing the entire LMNA gene, as well as the UBQLN4, MAPBPIP, and RAB25 genes was modified to insert the G608G HGPS mutation and a FRT site flanked kanamycin selection cassette into exon 11 of the LMNA gene. The kanamycin selection cassette was removed by transient Flp-recombinase expression. The resulting circular BAC transgene, which contains the G608G mutation in exon 11, and 109 extra nucleotides in intron 10 including an FRT site, EcoRI site, and SacI site, was microinjected into C57BL/6 donor eggs. Founder line H was subsequently established. (J:107176)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:107176 Varga R, et al., Progressive vascular smooth muscle cell defects in a mouse model of Hutchinson-Gilford progeria syndrome. Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3250-5
• All: 6 reference(s)