Rr20^tm1.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Rr20^tm1.1Vlcg
• Name: regulatory region 20; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5441845
• Synonyms: ECR5^-
• Gene: Rr20 Location: Chr11:101812178-101812508 bp Genetic Position: Chr11, Syntenic
• Alliance: Rr20^tm1.1Vlcg page

Higher bone mass in Rr20^tm1.1Vlcg/Rr20^tm1.1Vlcg and Sost^{tm1(KOMP)Vlcg}/Sost^{tm1(KOMP)Vlcg} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188586
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

Allele Type: Targeted (Modified regulatory region, Null/knockout) Mutations: Insertion, Intergenic deletion Mutation details: The evolutionarily conserved candidate enhancer element (ECR5) that acts as a distal enhancer to Sost was replaced with a floxed neo cassette. Cre-mediated recombination removed the neo cassette. Immunohistochemistry confirmed highly variable expression of Sost. (J:188586)

Rr20^tm1.1Vlcg/Rr20^tm1.1Vlcg mice have HBM due to reduced number of Sost-expressing osteocytes

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr20 Mutation: 0 strains or lines available

References

• Original: J:188586 Collette NM, et al., Targeted deletion of Sost distal enhancer increases bone formation and bone mass. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14092-7
• All: 2 reference(s)