Tcf21^{tm3.1(cre/Esr1*)Eno}
Targeted Allele Detail

Summary

• Symbol: Tcf21^{tm3.1(cre/Esr1*)Eno}
• Name: transcription factor 21; targeted mutation 3.1, Eric N Olson
• MGI ID: MGI:5442346
• Synonyms: Tcf21^iCre, Tcf21^MCM, Tcf21-MerCreMer
• Gene: Tcf21 Location: Chr10:22693162-22696042 bp, - strand Genetic Position: Chr10, 10.33 cM
• Alliance: Tcf21^{tm3.1(cre/Esr1*)Eno} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188837
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Tcf21^{tm3.1(cre/Esr1*)Eno} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tcf21 (MGI:1202715)

• Mutation details: A targeting vector was designed to replace exon 1 of the transcription factor 21,Tcf21 (capsulin; or epicardin) with a mercremer protein followed by a frt-flanked neo cassette. cre recombinase cDNA sequence flanked on each end with a mutated murine estrogen receptor (mer) ligand-binding domain. Flp-mediated recombination deletes the neo cassette. (J:188837)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tcf21 (mouse) Summary of all recombinase alleles driven by Tcf21. Alliance mouse Tcf21 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf21 Mutation: 16 strains or lines available

Notes

The construct was electroporated into 129SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice to establish a colony.These mice were subsequently bred to Tg(ACTFLPe)9205Dym mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene.

References

• Original: J:188837 Song K, et al., Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature. 2012 May 31;485(7400):599-604
• All: 33 reference(s)