Myd88^tm1Hlz
Targeted Allele Detail

Summary

• Symbol: Myd88^tm1Hlz
• Name: myeloid differentiation primary response gene 88; targeted mutation 1, Bernhard Holzmann
• MGI ID: MGI:5442766
• Synonyms: Myd88^LSL, MyD88^OFF
• Gene: Myd88 Location: Chr9:119165000-119169084 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Myd88^tm1Hlz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188968
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: An intron gene trap cassette, containing a loxP site, Engailed 2 splice acceptor/STOP (En-2/stp), IRES, lacZ, downstream element (DSE) and floxed neo cassette, was targeted to intron 1. This allele is both a knock-out and conditional-ready allele, as it can be reverted back to wild-type expression through cre-mediated recombination. Western blot analysis confirmed the absence of protein expression in bone marrow derived macrophage stimulated with (S)-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-N-palmitoyl-(R)-Cys-(S)-Ser-(S)-Lys4-OH (P3C). (J:188968)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Myd88^tm1Hlz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Myd88 Mutation: 52 strains or lines available

References

• Original: J:188968 Gais P, et al., Cutting edge: Divergent cell-specific functions of MyD88 for inflammatory responses and organ injury in septic peritonitis. J Immunol. 2012 Jun 15;188(12):5833-7
• All: 21 reference(s)