Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5, Jeremy Nathans
• MGI ID: MGI:5443817
• Synonyms: CAG-Sun1sfGFP, R26-CAG-LSL-Sun1-sfGFP-Myc, SUN1-2xsfGFP-6xMYC, Sun1-LsL, Sun1^LsL
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243557
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sun1 (MGI:1924303)

• Mutation details: A targeting vector containing (from 5' to 3') a CAG promoter, a loxP site flanked STOP cassette, the mouse Sun1 cDNA fused with 2 copies of the superfolder GFP (sfGFP) green fluorescent protein gene and 6 copies of the Myc tag, and an FRT site flanked neomycin resistance gene cassette was inserted into the locus. Removal of the floxed stop cassette is required to achieve expression. (J:243557)

Expression

In Mice Carrying this Mutation:

7 assay results 6 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:243557 Mo A, et al., Epigenomic Signatures of Neuronal Diversity in the Mammalian Brain. Neuron. 2015 Jun 17;86(6):1369-84
• All: 90 reference(s)