Hcn3^tm1.1Biel
Targeted Allele Detail

Summary

• Symbol: Hcn3^tm1.1Biel
• Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 3; targeted mutation 1.1, Martin Biel
• MGI ID: MGI:5443894
• Gene: Hcn3 Location: Chr3:89054082-89067538 bp, - strand Genetic Position: Chr3, 39.01 cM, cytoband F2
• Alliance: Hcn3^tm1.1Biel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188823
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An floxed neo cassette was inserted downstream of exon 2. Cre-mediated recombination removed exon 2 and the neo cassette. Western blot analysis confirmed the absence of protein expression in the brain. (J:188823)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hcn3 Mutation: 25 strains or lines available

References

• Original: J:188823 Fenske S, et al., HCN3 contributes to the ventricular action potential waveform in the murine heart. Circ Res. 2011 Oct 14;109(9):1015-23
• All: 5 reference(s)