Et(cre)TFC.09Lois
Transgene Detail

Summary

• Symbol: Et(cre)TFC.09Lois
• Name: enhancer trap TFC.09, Carlos Lois
• MGI ID: MGI:5443915
• Transgene: Et(cre)TFC.09Lois Location: unknown Genetic Position: ChrX, Syntenic
• Alliance: Et(cre)TFC.09Lois page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Et(cre)TFC.09Lois expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Et(cre)TFC.09Lois (MGI:5443913)

• Mutation details: Injection of a lentiviral vector carrying an enhancer trap detector cassette composed of Cre::GFP fusion gene under control of the mouse Thy1.2 minimal promoter (129 bp) was used to generate several cre lines. These were crossed with Z/EG reporter mice, and the patterns of GFP labeling in the brains of the offspring were examined. One line, TCF.09, displayed a low rate of recombination in a sparse and seemingly random subset of neural precursors during development. A single copy of the transgene integrated into the seventh intron of the glypican-3 gene; recombination activity showed no correlation with endogenous Gpc3 gene expression. The investigator reports that GFP expression is almost undetectable by fluorescence and it is only transiently expressed in a few stem cells in the embryo. (J:188479)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Et(cre)TFC.09Lois (mouse) Summary of all recombinase alleles driven by Et(cre)TFC.09Lois. Alliance mouse Et(cre)TFC.09Lois gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:188479 Magavi S, et al., Coincident generation of pyramidal neurons and protoplasmic astrocytes in neocortical columns. J Neurosci. 2012 Apr 4;32(14):4762-72
• All: 2 reference(s)