Eif4e2^tm1.1Nso
Targeted Allele Detail

Summary

• Symbol: Eif4e2^tm1.1Nso
• Name: eukaryotic translation initiation factor 4E member 2; targeted mutation 1.1, Nahum Sonenberg
• MGI ID: MGI:5444035
• Synonyms: 4ehp KO
• Gene: Eif4e2 Location: Chr1:87141636-87168210 bp, + strand Genetic Position: Chr1, 44.08 cM, cytoband C5
• Alliance: Eif4e2^tm1.1Nso page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188802
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and FRT flanked neo cassette were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 6 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 4 - 6. Immunoblot analysis confirmed the absence of protein expression in MEFs from homozygous mice. (J:188802)

Expression

In Mice Carrying this Mutation:	9 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eif4e2 Mutation: 93 strains or lines available

References

• Original: J:188802 Morita M, et al., A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development. Mol Cell Biol. 2012 Sep;32(17):3585-93
• All: 2 reference(s)