Tgs1^tm1.2Jkr
Targeted Allele Detail

Summary

• Symbol: Tgs1^tm1.2Jkr
• Name: trimethylguanosine synthase 1; targeted mutation 1.2, Janardan K Reddy
• MGI ID: MGI:5444606
• Synonyms: PIMT^-, PIMT^null
• Gene: Tgs1 Location: Chr4:3574875-3616619 bp, + strand Genetic Position: Chr4, 1.99 cM, cytoband A1
• Alliance: Tgs1^tm1.2Jkr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189008
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT flanked neo cassette and loxP site were inserted upstream of exon 3 and a loxP site was inserted downstream of exon 4. Flp and cre mediated recombination removed the neo cassette and exons 3 and 4. Immunostaining analysis confirmed the absence of protein expression on homozygous blastocysts at E3.5. (J:189008)

Expression

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgs1 Mutation: 31 strains or lines available

References

• Original: J:189008 Jia Y, et al., Early embryonic lethality of mice with disrupted transcription cofactor PIMT/NCOA6IP/Tgs1 gene. Mech Dev. 2012 Sep;129(9-12):193-207
• All: 1 reference(s)