Sftpc^{tm1(cre/ERT2,rtTA)Hap}
Targeted Allele Detail

Summary

• Symbol: Sftpc^{tm1(cre/ERT2,rtTA)Hap}
• Name: surfactant associated protein C; targeted mutation 2, Harold A Chapman
• MGI ID: MGI:5444645
• Synonyms: SPCcre-ER
• Gene: Sftpc Location: Chr14:70758389-70761521 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Sftpc^{tm1(cre/ERT2,rtTA)Hap} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175915
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 and C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Transactivator)
• Inducer: tamoxifen
• Mutation: Insertion
• Sftpc^{tm1(cre/ERT2,rtTA)Hap} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sftpc (MGI:109517)

• Mutation details: The terminal stop codonin exon 5 of the endogenous mouse gene was replaced with an estrogen-dependent cre cDNA (creERT2) preceded in frame by a E2A peptide sequence to interrupt translation between SPC and cre mRNAs coexpressed on a single transcript and followed by a T2A peptide sequence and rtTA. This was then followed by an Frt flanked neo selection cassette. (J:175915)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sftpc (mouse) Summary of all recombinase alleles driven by Sftpc. Alliance mouse Sftpc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sftpc Mutation: 24 strains or lines available

Notes

Targeting was accomplished in 129/C57bl6 hybrid ES cells (iGeniousTL, Inc., Stony Brook, NY).

References

• Original: J:175915 Chapman HA, et al., Integrin alpha6beta4 identifies an adult distal lung epithelial population with regenerative potential in mice. J Clin Invest. 2011 Jul 1;121(7):2855-62
• All: 50 reference(s)