Gt(ROSA)26Sor^{tm13(CAG-MYC,-CD2*)Rsky}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm13(CAG-MYC,-CD2*)Rsky}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 13, Klaus Rajewsky
• MGI ID: MGI:5444670
• Synonyms: MYC^stopFL, R26Stop^FLMYC
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm13(CAG-MYC,-CD2*)Rsky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188564
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm13(CAG-MYC,-CD2*)Rsky} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CD2 (914)

  Cd2	(MGI:88320)

  human

  MYC (4609)

  Bmyc	(MGI:88184)
  Myc	(MGI:97250)

• Mutation details: A splice acceptor site, CAG promoter, floxed neo stop cassette human MYC cDNA, and an FRT flanked IRES sequence and human CD2 cDNA were knocked into intron 1 via homologous recombination. The human CD2 cDNA is a signaling-deficient truncated version. (J:188564)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 992 strains or lines available

References

• Original: J:188564 Calado DP, et al., The cell-cycle regulator c-Myc is essential for the formation and maintenance of germinal centers. Nat Immunol. 2012 Nov;13(11):1092-100
• All: 21 reference(s)