Abcg2^{tm3.1(cre/ERT2)Bsor}
Targeted Allele Detail

Summary

• Symbol: Abcg2^{tm3.1(cre/ERT2)Bsor}
• Name: ATP binding cassette subfamily G member 2 (Junior blood group); targeted mutation 3.1, Brian P Sorrentino
• MGI ID: MGI:5444690
• Synonyms: Abcg2^CreERT2, Abcg2-Ires-CreERT2
• Gene: Abcg2 Location: Chr6:58561476-58669436 bp, + strand
• Alliance: Abcg2^{tm3.1(cre/ERT2)Bsor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189206
• Parent Cell Line: F12 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Hypomorph, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Abcg2^{tm3.1(cre/ERT2)Bsor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Abcg2 (MGI:1347061)

• Mutation details: A targeting vector was generated with a neomycin resistance cassette, IRES-cre/ERT2 and polyadenylation sequences. The construct was electroporated into ES cells, and homologous recombination inserted the neo-IRES-cre/ERT2-pA sequence into exon 16 of the Abcg2 gene. Correctly targeted ES cells were transiently transfected with a pMC-cre plasmid to excise the nomycin cassette. The ES cells were injected into blastocysts to generate chimeric animals which were used to establish germline transmission. The allele is stated to be hypomorphic for Abcg2 protein expression in the kidney (slight reduction) and liver (greater reduction) determined by Western blot, in homozygous animals. (J:189206)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Abcg2 (mouse) Summary of all recombinase alleles driven by Abcg2. Alliance mouse Abcg2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Abcg2 Mutation: 76 strains or lines available

References

• Original: J:189206 Fatima S, et al., Abcg2 expression marks tissue-specific stem cells in multiple organs in a mouse progeny tracking model. Stem Cells. 2012 Feb;30(2):210-21
• All: 7 reference(s)