Grhl3^{tm2.1(F2rl1)Cgh}
Targeted Allele Detail

Summary

• Symbol: Grhl3^{tm2.1(F2rl1)Cgh}
• Name: grainyhead like transcription factor 3; targeted mutation 2.1, Shaun R Coughlin
• MGI ID: MGI:5444857
• Synonyms: Grhl3^Par2
• Gene: Grhl3 Location: Chr4:135269199-135300941 bp, - strand Genetic Position: Chr4, 67.76 cM
• Alliance: Grhl3^{tm2.1(F2rl1)Cgh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189278
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A cDNA encoding the mouse F2rl1 was inserted in frame into the ATG start codon. An internal ribosomal entry sequence (IRES) followed by a nuclear localization signal and lacZ gene were introduced downstream of the F2rl1 gene. An FRT-flanked Neo cassette was positioned 5' of the F2rl1. Chimeric males derived from blastocyst injections of positive clones into C57BL/6 recipients were mated with C57BL/6 FLPe females to remove the neo gene. (J:189278)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grhl3 Mutation: 53 strains or lines available

References

• Original: J:189278 Frateschi S, et al., PAR2 absence completely rescues inflammation and ichthyosis caused by altered CAP1/Prss8 expression in mouse skin. Nat Commun. 2011 Jan 18;2:161
• All: 4 reference(s)