Raf1^tm2.1Ara
Targeted Allele Detail

Summary

• Symbol: Raf1^tm2.1Ara
• Name: v-raf-leukemia viral oncogene 1; targeted mutation 2.1, Toshiyuki Araki
• MGI ID: MGI:5445964
• Synonyms: Raf1^D486N
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^tm2.1Ara page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189143
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A targeting vector containing a loxP site, cDNA spanning exons 13 through 16, neo cassette, second loxP site and modified exon 13 was inserted into intron 12. The modified exon 13 contains nucleotide substitution(s) that result in the amino acid substitution of asparagine for aspartic acid at position 486 (D486N). Cre-mediated recombination removed the cDNA and neo cassette. (J:189143)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Raf1 Mutation: 117 strains or lines available

References

• Original: J:189143 Wu X, et al., Increased BRAF heterodimerization is the common pathogenic mechanism for noonan syndrome-associated RAF1 mutants. Mol Cell Biol. 2012 Oct;32(19):3872-90
• All: 1 reference(s)