Nsf^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Nsf^{tm1b(KOMP)Mbp}
• Name: N-ethylmaleimide sensitive fusion protein; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:5446014
• Gene: Nsf Location: Chr11:103712608-103844882 bp, - strand Genetic Position: Chr11, 67.54 cM
• Alliance: Nsf^{tm1b(KOMP)Mbp} page
• IMPC: Nsf gene page

Mutation origin

• Mutant Cell Line: DEPD00520_2_A09
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 103803876 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 103805154. The critical exon(s) were thus flanked by loxP sites, subsequent cre expression then excised this critical sequence resulting in a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157064, J:188991)

Expression

In Mice Carrying this Mutation:	44 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nsf Mutation: 153 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 5 reference(s)