Rasa1^tm1Wid
Targeted Allele Detail

Summary

• Symbol: Rasa1^tm1Wid
• Name: RAS p21 protein activator 1; targeted mutation 1, Christian Widmann
• MGI ID: MGI:5446031
• Synonyms: RasGAP^D455A
• Gene: Rasa1 Location: Chr13:85362899-85437249 bp, - strand Genetic Position: Chr13, 45.05 cM
• Alliance: Rasa1^tm1Wid page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189241
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 10 was replaced with a modified one in which nucleotide substitutions (GAT GGG to GCC GGC) results in the amino acid substitution of alanine for aspartic acid at position 455 (D455A). This modification is predicted to produce a caspase-resistant protein. A floxed neo cassette was inserted downstream of the modified exon 10. (J:189241)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasa1 Mutation: 34 strains or lines available

References

• Original: J:189241 Khalil H, et al., Caspase-3 Protects Stressed Organs against Cell Death. Mol Cell Biol. 2012 Nov;32(22):4523-33
• All: 2 reference(s)