Nras^tm1.1Emfu
Targeted Allele Detail

Summary

• Symbol: Nras^tm1.1Emfu
• Name: neuroblastoma ras oncogene; targeted mutation 1.1, Ernst-Martin Fuchtbauer
• MGI ID: MGI:5447943
• Synonyms: LTR, LTR9NS, Nras^LTR9S
• Gene: Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Nras^tm1.1Emfu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189834
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

Allele Type: Targeted (Not Applicable) Mutation: Insertion Mutation details: A floxed PGk/Tn4 neo cassette and the Akv1-99 murine leukemia virus long terminal repeat were inserted into intron 1 via homologous recombination. The viral long terminal repeat was inserted at the proviral integration site before nucleotide 1062 of the GenBank sequence no. L19607. Cre mediated recombination removed the neo cassette. Q-RT-PCR analysis indicated increased mRNA expression in most tissues in heterozygous and homozygous mice. Western blot analysis confirmed increased protein expression. (J:189834)

Schematic of the Nras^tm1.1Emfu allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nras Mutation: 44 strains or lines available

References

• Original: J:189834 Lassen LB, et al., Nras overexpression results in granulocytosis, T-cell expansion and early lethality in mice. PLoS One. 2012;7(8):e42216
• All: 2 reference(s)