Vsx2^tm1.1Itl
Targeted Allele Detail

Summary

• Symbol: Vsx2^tm1.1Itl
• Name: visual system homeobox 2; targeted mutation 1.1, inGenious Targeting Laboratory
• MGI ID: MGI:5449355
• Synonyms: R200Q, Vsx2^R200Q
• Gene: Vsx2 Location: Chr12:84616602-84642231 bp, + strand Genetic Position: Chr12, 39.28 cM
• Alliance: Vsx2^tm1.1Itl page

Small eyes in various Vsx2 homozygous mutants

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:190452
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Nucleotide substitutions Mutation details: A self excising selection cassette (ACN) was inserted in intron 3 and the bases agg were changed to cgc in exon 4 via homologous recombination. The base changes result in an arginine to glutamine substitution at position 200 (R200Q). Cre mediated recombination in the male germ line removed the selection cassette. Protein expression is similar to wild-type protein but DNA binding is absent. (J:190452)

Generation of the Vsx2^tm1.1Itl and Vsx2^tm1.1Eml alleles

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vsx2 Mutation: 23 strains or lines available

References

• Original: J:190452 Zou C, et al., Vsx2 controls eye organogenesis and retinal progenitor identity via homeodomain and non-homeodomain residues required for high affinity DNA binding. PLoS Genet. 2012 Sep;8(9):e1002924
• All: 1 reference(s)