Gt(ROSA)26Sor^{tm1(CAG-PA-GFP)Rmpl}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-PA-GFP)Rmpl}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Simon Rumpel
• MGI ID: MGI:5449399
• Synonyms: R26 PA-GFP::NLS
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-PA-GFP)Rmpl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202089
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The R26 PA-GFP::NLS targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked neomycin-beta-galactosidase fusion protein (betageo) with a STOP codon, a photoactivatable green fluorescent protein (PA-GFP) fused to a nuclear localization signal (NLS), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability). (J:202089)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

Notes

After removal of the loxP-flanked cassette via cre-mediated recombination, PA-GFP is expressed in all cells where Cre recombinase is expressed. PA-GFP::NLS is enriched in the nucleus, but can be also detected in the cytoplasm of neuronal cells. PA-GFP shows very weak fluorescence at 517nm when excited at 488nm before photoactivation. Upon photoactivation with light at ~400nm PA-GFP is converted into its fluorescent form. This form shows strong emission at 517nm when excited at 488nm and allows the conditional fluorescence labeling of individual, living cells which can be imaged using conventional GFP filter sets. Photoactivation can also be efficiently achieved using 2-photon excitation at ~750nm and imaged after photoconversion using excitation at ~950nm.

References

• Original: J:202089 Peter M, et al., Transgenic mouse models enabling photolabeling of individual neurons in vivo. PLoS One. 2013;8(4):e62132
• All: 5 reference(s)