Hprt1^{tm1(CMV-GFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(CMV-GFP)Nat}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:5449926
• Synonyms: CAG-LSL-nlsGFP, Hprt^{tm1(CMV-LSL-nlsGFP)Nat}
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(CMV-GFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220411
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A pair of insulator sequences, CMV enhancer/beta-actin promoter, loxP site, 3 stop transcription sites, loxP site, nuclear localized (nls) GFP-6xMyc epitope, rabbit beta-globin 3' UTR, and a pair of insulator sequences were inserted into the gene in the indicated order via targeted recombination. (J:220411)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:220411 Wu H, et al., Cellular resolution maps of X chromosome inactivation: implications for neural development, function, and disease. Neuron. 2014 Jan 8;81(1):103-19
• All: 2 reference(s)