Cx3cr1^{tm2.1(cre/ERT2)Litt}
Targeted Allele Detail

Summary

• Symbol: Cx3cr1^{tm2.1(cre/ERT2)Litt}
• Name: C-X3-C motif chemokine receptor 1; targeted mutation 2.1, Dan R Littman
• MGI ID: MGI:5450813
• Synonyms: Cx3cr1^CreER, CX3CR1^YFP-creER
• Gene: Cx3cr1 Location: Chr9:119877749-119897362 bp, - strand Genetic Position: Chr9, 71.37 cM
• Alliance: Cx3cr1^{tm2.1(cre/ERT2)Litt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205483
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Cx3cr1^{tm2.1(cre/ERT2)Litt} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cx3cr1 (MGI:1333815)

• Mutation details: A targeting vector was designed to replace exon 2 of the chemokine (C-X3-C) receptor 1 (Cx3cr1) gene with a cre/ERT2 (cre recombinase fused to an estrogen receptor ligand binding domain) coding sequence, followed by an internal ribosome entry site (IRES) and an enhanced yellow fluorescent protein (EYFP). A frt-flanked neomycin resistance cassette, in reverse orientation to the gene, was also inserted downstream of the EYFP sequence. Flp-mediated recombination excised the neomycin resistance sequence. (J:190965, J:205483)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cx3cr1 (mouse) Summary of all recombinase alleles driven by Cx3cr1. Comparative matrix view of recombinase activities Alliance mouse Cx3cr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cx3cr1 Mutation: 45 strains or lines available

References

• Original: J:190965 Littman DR, An inducible cre recombinase driven by Cx3cr1. MGI Direct Data Submission. 2013
• All: 262 reference(s)