Pvalb^{tm1.1(cre/ERT2)Hze}
Targeted Allele Detail

Summary

• Symbol: Pvalb^{tm1.1(cre/ERT2)Hze}
• Name: parvalbumin; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5461312
• Synonyms: Pvalb-2A-CreERT2, Pvalb-2A-CreERT2-Deltahygro, Pvalb^{tm1.1(cre/ERT2)Htz}
• Gene: Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
• Alliance: Pvalb^{tm1.1(cre/ERT2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191262
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Pvalb^{tm1.1(cre/ERT2)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pvalb (MGI:97821)

• Mutation details: Embryonic stem (ES) cells, previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a T2A-CreERT2 vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a CreERT2 fusion gene, the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the attB/attP-flanked PGK cassette. The resulting mice were bred with C57BL/6J wildtype mice for at least two additional generations (and the PhiC31 transgene was removed). (J:155793, J:191262, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pvalb (mouse) Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities Alliance mouse Pvalb gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pvalb Mutation: 29 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 7 reference(s)