Gt(ROSA)26Sor^{tm40.1(CAG-aop3/EGFP)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm40.1(CAG-aop3/EGFP)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 40.1, Hongkui Zeng
• MGI ID: MGI:5461316
• Synonyms: Ai40D, Ai40Deltaneo, Rosa-CAG-LSL-ArchT/EGFP-WPRE-bGHpA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm40.1(CAG-aop3/EGFP)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191265
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-pCAG-LSL-ArchT/EGFP-WPRE-bGHpA-AttB-PGK-frt-Neo-polyA-AttP targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an frt site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames linked to three repeats of an SV40 polyA signal), an ArchT-EGFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, an attB site, a PGK promoter, a second frt site, neomycin resistance gene-SV40polyA signal, and an attP site. The ArchT-EGFP fusion gene is a mammalian codon-optimized cDNA sequence encoding the Archaerhodopsin-3 protein derived from the halophilic bacterium Halorubrum strain TP009 (ArchT or aR-TP009) fused in-frame via a 15 nucleotide linker to the amino terminus of an enhanced green fluorescent protein (EGFP). PhiC31-mediated recombination removed the attB/attP-flanked PGK-frt-Neo-polyA cassette and left a recombined attB/attP site (attL). (J:191265)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:191265 Zeng H, Direct Data Submission. MGI Direct Data Submission. 2013
• All: 58 reference(s)