Ngf^tm1.1Blhe
Targeted Allele Detail

Summary

• Symbol: Ngf^tm1.1Blhe
• Name: nerve growth factor; targeted mutation 1.1, Barbara L Hempstead
• MGI ID: MGI:5461488
• Synonyms: proNgf-HA
• Gene: Ngf Location: Chr3:102377235-102428329 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Ngf^tm1.1Blhe page

Adult Ngf^tm1.1Blhe/Ngf⁺ mice exhibit dilated cardiomyopathy and fibrosis and this phentoype is rescued in Ngfr^tm1Jae/Ngfr^tm1Jae Ngf^tm1.1Blhe/Ngf⁺ mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:190893
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Nucleotide substitutions result in the amino acid substitution of lysine and arginine for two alanines at positions 120 and 121 within the furin recognition site. Cre-mediated recombination removed the FRT- and loxP-flanked neo cassette inserted upstream of the modified exon. An HA-tag was inserted in-frame before the stop codon. (J:190893)

Generation of the Ngf^tm1.1Blhe allele

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ngf Mutation: 12 strains or lines available

References

• Original: J:190893 Siao CJ, et al., ProNGF, a cytokine induced after myocardial infarction in humans, targets pericytes to promote microvascular damage and activation. J Exp Med. 2012 Nov 19;209(12):2291-305
• All: 1 reference(s)