Ebf2^{tm3(Zfp423*)Reed}
Targeted Allele Detail

Summary

• Symbol: Ebf2^{tm3(Zfp423*)Reed}
• Name: early B cell factor 2; targeted mutation 3, Randall R Reed
• MGI ID: MGI:5461559
• Synonyms: O/E3^{OAZdeltaC-LNL}
• Gene: Ebf2 Location: Chr14:67470741-67668367 bp, + strand Genetic Position: Chr14, 34.63 cM, cytoband D-E1
• Alliance: Ebf2^{tm3(Zfp423*)Reed} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:190934
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing a full length cDNA of Zfp423 with a single nucleotide insertion (3877_3878insT) between ZF 27 and ZF 28, corresponding to amino acid 1208 of the protein, followed by an IRES, YFP and a floxed neo selection cassette was inserted into the 5' UTR, replacing the first five exons of the locus. The insertion creates a termination codon and altered reading frame in all reading frames. Presence of the neo cassette severely impairs expression of the inserted construct. (J:190934)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ebf2 Mutation: 30 strains or lines available

References

• Original: J:190934 Roby YA, et al., Zfp423/OAZ mutation reveals the importance of Olf/EBF transcription activity in olfactory neuronal maturation. J Neurosci. 2012 Oct 3;32(40):13679-88a
• All: 1 reference(s)