Tg(Prnp-TARDBP)96Dwc
Transgene Detail

Summary

• Symbol: Tg(Prnp-TARDBP)96Dwc
• Name: transgene insertion 96, Don W Cleveland
• MGI ID: MGI:5463113
• Synonyms: TDP-43^WT
• Transgene: Tg(Prnp-TARDBP)96Dwc Location: unknown
• Alliance: Tg(Prnp-TARDBP)96Dwc page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-TARDBP)96Dwc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TARDBP (23435)

  Tardbp

  (MGI:2387629)

• Mutation details: A full-length human TAR DNA binding protein (TARDBP or TDP-43) cDNA sequence was modified to have an N-terminal myc tag. This huTDP-43-WT cDNA sequence was inserted between exon 2 and exon 3 of mouse prion protein (Prnp) gene. The resulting Prnp-TDP-43-WT transgene was flanked with loxP sites. Three lines (65 93 and 96) were produced. Founder line 96 was identified with about 1.5-fold greater total TDP-43 expression levels in spinal cord as compared to endogenous TDP-43 in non-transgenic mice. (J:191785)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:191785 Arnold ES, et al., ALS-linked TDP-43 mutations produce aberrant RNA splicing and adult-onset motor neuron disease without aggregation or loss of nuclear TDP-43. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):E736-45
• All: 7 reference(s)