Rr21^tm1.1Jta
Targeted Allele Detail

Summary

• Symbol: Rr21^tm1.1Jta
• Name: regulatory region 21; targeted mutation 1.1, Jussi Taipale
• MGI ID: MGI:5463413
• Gene: Rr21 Location: Chr15:61490136-61491875 bp Genetic Position: Chr15, Syntenic
• Alliance: Rr21^tm1.1Jta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191217
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A 1740 bp region that lies 335 kb upstream of the transcription start site of Myc (Chr15:61449842-61451581 (NCBI37/mm9)) was flanked with loxP sites and an FRT site flanked neomycin resistance gene cassette was inserted upstream of the 3' loxP site. Cre-mediated recombination removed the 1740 bp region and the neo cassette, leaving a single loxP site at chr15:61490136 (GRCm39). Expression of Myc RNA was decreased in the colon but not duodenum of homozygous mice at P1. Chromatin immunoprecipitation analysis indicated the loss of TCF7L2 binding in this region. (J:191217)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr21 Mutation: 0 strains or lines available

References

• Original: J:191217 Sur IK, et al., Mice lacking a Myc enhancer that includes human SNP rs6983267 are resistant to intestinal tumors. Science. 2012 Dec 7;338(6112):1360-3
• All: 2 reference(s)