Tg(Thy1-APPArc)M8Cagr
Transgene Detail

Summary

• Symbol: Tg(Thy1-APPArc)M8Cagr
• Name: transgene insertion M8, Caroline Graff
• MGI ID: MGI:5463435
• Synonyms: TgAPParc, Thy1.2-hAPParc
• Transgene: Tg(Thy1-APPArc)M8Cagr Location: unknown
• Alliance: Tg(Thy1-APPArc)M8Cagr page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-APPArc)M8Cagr expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene encodes for the Arctic mutation.

• Mutation details: The Arctic mutation, an A>G nucleotide substitution resulting in a glutamic acid to glycine amino acid substitution at codon 693 (E693G), was introduced into a human cDNA of APP (695 isoform) by site-directed mutagenesis and a Kozak sequence was introduced upstream of ATG to enhance translation. This construct was placed under the control of the mouse Thy1.2 expression cassette that drives expression of the transgene specifically in neurons. Two lines were made, M7 and M8. M8 is the high expressor line and hemizygous mice have an approximately 3-fold higher expression of human APP compared to endogenous App. (J:168288)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:168288 Ronnback A, et al., Progressive neuropathology and cognitive decline in a single Arctic APP transgenic mouse model. Neurobiol Aging. 2011 Feb;32(2):280-92
• All: 4 reference(s)