Cenpo^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Cenpo^{tm1b(KOMP)Mbp}
• Name: centromere protein O; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:5463969
• Synonyms: Cenpo^-
• Gene: Cenpo Location: Chr12:4246004-4284294 bp, - strand Genetic Position: Chr12, 2.02 cM, cytoband A1.1
• Alliance: Cenpo^{tm1b(KOMP)Mbp} page
• IMPC: Cenpo gene page

Cenpo^{tm1b(KOMP)Mbp}/Cenpo^{tm1b(KOMP)Mbp} mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos are smaller and form a rudimentary egg cylinder at E7.5, but no primitive streak or hallmarks of gastrulation are seen indicating arrest prior to gastrulation.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: DEPD00518_3_C02
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4265061 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4267761. The critical exon(s) were thus flanked by loxP sites and subsequent expression of cre recombinase then excised this critical sequence resulting in a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:188991)

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cenpo Mutation: 28 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 7 reference(s)