Chpf^tm1.2Hwat
Targeted Allele Detail

Summary

• Symbol: Chpf^tm1.2Hwat
• Name: chondroitin polymerizing factor; targeted mutation 1.2, Hideto Watanabe
• MGI ID: MGI:5464019
• Gene: Chpf Location: Chr1:75451213-75455951 bp, - strand Genetic Position: Chr1, 39.14 cM
• Alliance: Chpf^tm1.2Hwat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191659
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 1. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 1. Flp-mediated recombination removed the neo cassette and cre-mediated recombination removed exon 1. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:191659)

Generation of the Chpf^tm1.1Hwat and Chpf^tm1.2Hwat alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chpf Mutation: 30 strains or lines available

References

• Original: J:191659 Ogawa H, et al., Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development. PLoS One. 2012;7(8):e43806
• All: 1 reference(s)