Tg(Serpinb7-icre)1Fech
Transgene Detail

Summary

• Symbol: Tg(Serpinb7-icre)1Fech
• Name: transgene insertion 1, Feng Chen
• MGI ID: MGI:5464108
• Synonyms: Megsin-cre
• Transgene: Tg(Serpinb7-icre)1Fech Location: unknown
• Alliance: Tg(Serpinb7-icre)1Fech page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Serpinb7-icre)1Fech expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Serpinb7

• Mutation details: A BAC clone containing only the Serpinb7 (Megsin) gene was modifed by recombineering methods to generate the construct. The first coding exon was replaced with the sequence encoding codon-improved cre recombinase (icre) fused to a nuclear localization signal (nls) with a Myc tag. An FRT-flanked ampicillin selection cassette was also inserted. Transient FLP expression in bacterial cells removed the amp cassette from the construct. The final construct was purified, sequenced and injected into pronuclei of fertilized oocytes to generate transgenic mice. One fertile founder was identified and cre specificity was assessed by crossing the line to reporter animals. Recombination was observed in the epidermal layers of the skin and hair follicles, and epithelium of forestomach, and esophagus, but, unexpectedly, activity was not observed in kidney mesangial cells. (J:191899)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Serpinb7 (species not specified) Summary of all recombinase alleles driven by Serpinb7.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:191899 Wang Y, et al., A new tool for conditional gene manipulation in a subset of keratin-expressing epithelia. Genesis. 2012 Dec;50(12):899-907
• All: 1 reference(s)