Tg(Cd4-cre/ERT2)11Gnri
Transgene Detail

Summary

• Symbol: Tg(Cd4-cre/ERT2)11Gnri
• Name: transgene insertion 11, Fotini Gounari
• MGI ID: MGI:5464279
• Synonyms: CD4CreER^T2
• Transgene: Tg(Cd4-cre/ERT2)11Gnri Location: unknown
• Alliance: Tg(Cd4-cre/ERT2)11Gnri page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Cd4-cre/ERT2)11Gnri expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Cd4

• Mutation details: A fragment encoding the cre recombinase fused to a mutated ligand-binding domain of the estrogen receptor (ERT2) was cloned downstream of the Cd4 gene promoter/enhancer/silencer to generate the transgene construct. The construct also contained the human growth hormone (hGH) polyadenylation sequence. Pronuclei of fertilized eggs were microinjected with the purified construct. Founder lines 11 and 19 were established. These were crossed to R26R-EYFP reporter mice. CD4 T cells were isolated from tamoxifen-treated double mutant animals and 50 and 30% of cells analyzed by FACS from lines 11 and 19 respectively showed EYFP expression. Line 11 had greater recombinase activity and was used for further analyses. (J:191897)

Generation of the Tg(Cd4-cre/ERT2)11Gnri transgene.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cd4 (species not specified) Summary of all recombinase alleles driven by Cd4.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:191897 Aghajani K, et al., Generation of CD4CreER(T2) transgenic mice to study development of peripheral CD4-T-cells. Genesis. 2012 Dec;50(12):908-13
• All: 47 reference(s)