Sox2^tm1.2Vlcg
Targeted Allele Detail

Summary

• Symbol: Sox2^tm1.2Vlcg
• Name: SRY (sex determining region Y)-box 2; targeted mutation 1.2, Velocigene
• MGI ID: MGI:5464909
• Synonyms: Sox2^INV
• Gene: Sox2 Location: Chr3:34704554-34706610 bp, + strand Genetic Position: Chr3, 16.93 cM, cytoband A2-B
• Alliance: Sox2^tm1.2Vlcg page

Sox2^tm1.2Vlcg/Sox2^tm1.2Vlcg and Sox2^tm1.1Vep/Sox2^tm1.2Vlcg embryos form disorganized extraembryonic tissues, but fail to form the epiblast, phenocopying Sox2^tm1.1Vep/Sox2^tm1.1Vep embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191980
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutation: Insertion Mutation details: The COIN module (L71 loxP site, inverted polyadenylation sequence, inverted EGFP, L66 loxP site and FRT-flanked neo cassette) was inserted after the thirtieth nucleotide of the coding exon. This insertion divides the CpG island. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination restored the orientation of the EGFP to allow for expression. Western blot analysis confirmed a reduction in protein expression in heterozygous samples. (J:191980)

Generation of the Sox2^tm1.1Vlcg and Sox2^tm1.2Vlcg alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox2 Mutation: 56 strains or lines available

References

• Original: J:191980 Mandalos N, et al., Application of a novel strategy of engineering conditional alleles to a single exon gene, Sox2. PLoS One. 2012;7(9):e45768
• All: 2 reference(s)