Crb2^tm1.1Wij
Targeted Allele Detail

Summary

• Symbol: Crb2^tm1.1Wij
• Name: crumbs family member 2; targeted mutation 1.1, Jan Wijnholds
• MGI ID: MGI:5466324
• Synonyms: Crb2^flox
• Gene: Crb2 Location: Chr2:37666261-37689115 bp, + strand Genetic Position: Chr2, 24.19 cM
• Alliance: Crb2^tm1.1Wij page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191149
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site and FRT flanked neomycin selection cassette were inserted in intron 9a and a second loxP site was inserted in exon 13 in the 3' untranslated region. The selection cassette was subsequently removed from the properly targeted allele using flp recombinase. Exons 10 through the coding part of exon 13 are then flanked by loxP sites. Cre excision will remove both the transmembrane domain and the C-terminal intracellular domain which includes the FERM and the PDZ protein binding domains. (J:191149)

Disease models

Expression

In Mice Carrying this Mutation:	119 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crb2 Mutation: 53 strains or lines available

References

• Original: J:191149 Alves CH, et al., Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene. Hum Mol Genet. 2013 Jan 1;22(1):35-50
• All: 13 reference(s)