Hormad2^tm1Kura
Targeted Allele Detail

Summary

• Symbol: Hormad2^tm1Kura
• Name: HORMA domain containing 2; targeted mutation 1, Hiroki Kurahashi
• MGI ID: MGI:5466566
• Gene: Hormad2 Location: Chr11:4295814-4391105 bp, - strand Genetic Position: Chr11, 2.94 cM, cytoband A1
• Alliance: Hormad2^tm1Kura page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:192634
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette replaced most of exon 3. Western blot analysis confirmed the absence of protein expression in the testes. (J:192634)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Hormad2^tm1Kura/Hormad2^tm1Kura	either: B6.Cg-Hormad2^tm1Kura or (involves: C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj)
cx2	Hormad2^tm1Kura/Hormad2^+ Spo11^tm1Mjn/Spo11^tm1Mjn	involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj
cx3	Hormad2^tm1Kura/Hormad2^tm1Kura Spo11^tm1Mjn/Spo11^tm1Mjn	involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj

Phenotypes:

Affected Systems	hm1		cx2	cx3
show or hide all annotated terms Sex:
cellular	√	√	√	√
decreased oocyte number			√
azoospermia		√
abnormal female meiosis	√		√	√
abnormal male meiosis		√
abnormal male germ cell apoptosis		√
endocrine/exocrine glands		√			√
abnormal testis morphology					√
small testis		√
decreased testis weight		√
reproductive system	√	√	√	√	√
reproductive system phenotype	N		N	N
decreased oocyte number			√
azoospermia		√
abnormal female meiosis	√		√	√
abnormal male meiosis		√
abnormal male germ cell apoptosis		√
abnormal testis morphology					√
small testis		√
decreased testis weight		√
male infertility		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hormad2 Mutation: 33 strains or lines available

References

• Original: J:192634 Kogo H, et al., HORMAD2 is essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity. Genes Cells. 2012 Nov;17(11):897-912
• All: 2 reference(s)