Cx3cr1^{tm2.1(cre/ERT2)Jung}
Targeted Allele Detail

Summary

• Symbol: Cx3cr1^{tm2.1(cre/ERT2)Jung}
• Name: C-X3-C motif chemokine receptor 1; targeted mutation 2.1, Steffen Jung
• MGI ID: MGI:5467985
• Synonyms: Cx3cr1^creER
• Gene: Cx3cr1 Location: Chr9:119877749-119897362 bp, - strand Genetic Position: Chr9, 71.37 cM
• Alliance: Cx3cr1^{tm2.1(cre/ERT2)Jung} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193038
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Cx3cr1^{tm2.1(cre/ERT2)Jung} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cx3cr1 (MGI:1333815)

• Mutation details: A cre/ERT2 cassette with a floxed neo gene was inserted by RedE/T recombineering into a BAC containing the Cx3cr1 locus, replacing the coding exon of Cx3cr1. Properly targeted ES cells were aggregated with 8-cell embryos to generate chimeras. Chimeras were backcrossed to C57BL/6 mice. Tne neo was excised by crossing the animals to a cre deleter strain (Tg(Pgk1-cre)1Lni). (J:193038)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cx3cr1 (mouse) Summary of all recombinase alleles driven by Cx3cr1. Comparative matrix view of recombinase activities Alliance mouse Cx3cr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cx3cr1 Mutation: 45 strains or lines available

References

• Original: J:193038 Yona S, et al., Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis. Immunity. 2013 Jan 24;38(1):79-91
• All: 226 reference(s)