Gt(ROSA)26Sor^{tm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bruce Conklin
• MGI ID: MGI:5468098
• Synonyms: R26(EF1a-tTA/TetO-mCh-Rs1), TetO-mCh-Rs1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193162
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HTR4 (3360)

  Htr4	(MGI:109246)

• Mutation details: A combined EF1a-tTA and TetO-mCh/Rs1 transgene in a single construct (separated by a 2A ribosomal skip site) was inserted in the Gt(ROSA)26Sor locus. Rs1 is an engineered Gs-coupled G-protein coupled receptor in which an aspartic acid to alanine amino acid residue change at position 100 was made to generate serotonin-insensitive GPCR. (J:193162)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:193162 Hsiao EC, et al., Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Stem Cell Res Ther. 2011;2(2):11
• All: 1 reference(s)