Rasa3^tm1a(KOMP)J
Targeted Allele Detail

Summary

• Symbol: Rasa3^tm1a(KOMP)J
• Name: RAS p21 protein activator 3; targeted mutation 1a, Jackson
• MGI ID: MGI:5469322
• Gene: Rasa3 Location: Chr8:13617218-13727590 bp, - strand Genetic Position: Chr8, 6.29 cM
• Alliance: Rasa3^tm1a(KOMP)J page

Mutation origin

• Mutant Cell Lines: JKOMP47947-1F2, JKOMP47947-2A11
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The HTGRS1006_A_4_H03 targeting vector was obtained from The International Knockout Mouse Consortium by The Jackson Laboratory. The L1L2_Pgk_P cassette was inserted at position 13615101 of Chromosome 8 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 13614300. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasa3 Mutation: 62 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 4 reference(s)