Col1a1^{tm1(tetO-CTNNB1)Tcd}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-CTNNB1)Tcd}
• Name: collagen, type I, alpha 1; targeted mutation 1, TC Dale
• MGI ID: MGI:5469323
• Synonyms: Tet-OdeltaN89beta-catenin
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-CTNNB1)Tcd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193365
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-CTNNB1)Tcd} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CTNNB1 (1499)

  Ctnnb1

  (MGI:88276)

• Col1a1^{tm1(tetO-CTNNB1)Tcd} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RCME on KH2 cells inserted a cassette that contains the tetracycline response element driving inducible expression of the truncated human cDNA (deltaN89) with MYC tag into the 3'UTR. (J:193365)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:193365 Jarde T, et al., In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system. Oncogene. 2013 Feb 14;32(7):883-93
• All: 2 reference(s)